| 质粒名称: | pCytERM-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000764 |
| 产品用途: | Expresses CytERM-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | CytERM-mScarlet3-H |
| 物种来源: |
H.sapiens, Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pCytERM-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-mScarlet3-H (aka mYongHong)载体质粒基本信息
pCytERM-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pCytERM-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pCytERM-mScarlet3-H (aka mYongHong)质粒载体简介
pCytERM-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pCytERM-mScarlet3-H (aka mYongHong) 4782 bp DNA circular SYN 20-JUL-2026
DEFINITION TSN20241016-0571-00013_764_20241017-BAN40_A01_A01_i14.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4782)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4782
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 24..159
/label=SV40 ori
/note="SV40 origin of replication"
CDS 208..1002
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 1234..1281
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1610..2198
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 2373..2676
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2677..2880
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2932..3018
/codon_start=1
/label=cytochrome p450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
regulatory 3064..3073
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3070..3759
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 3882..4003
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4010..4465)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4492..4596
/gene="bla"
/label=AmpR promoter
promoter join(4737..4782,1..165)
/label=SV40 promoter
/note="SV40 early promoter"
promoter join(4759..4782,1..173)
/label=SV40 promoter
/note="SV40 early promoter"
ORIGIN
1 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
61 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
121 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
181 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
241 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
301 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
361 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
421 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
481 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
541 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
601 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
661 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
721 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
781 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
841 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
901 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
961 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
1021 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
1081 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
1141 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
1201 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
1261 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
1321 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
1381 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
1441 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
1501 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
1561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
1621 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
1681 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
1741 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
1801 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
1861 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
1921 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
1981 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
2041 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
2101 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
2161 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
2221 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
2281 attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg
2341 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
2401 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
2461 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
2521 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2581 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2641 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
2701 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
2761 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
2821 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
2881 ggtttagtga accgtcagat ccgctagcgc taccggactc agatctccac catggatcct
2941 gtggtggtgc tggggctctg tctctcctgt ttgcttctcc tttcactctg gaaacagagc
3001 tatgggggag ggaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
3061 gtcgccacca tggatagcac cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac
3121 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
3181 tacgagggca cccagaccgc caagctgagg gtgaccaagg gtggccccct gcccttctcc
3241 tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcacgaa gcaccccgcc
3301 gacatccccg actactggaa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg
3361 aacttcgagg acggcggcgc cgtgtccgtg gcccaggaca cctccctgga ggacggcacc
3421 ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag
3481 aagaagacaa tgggctggga agcatccacc gagcggttgt accccgagga cgtcgtgctg
3541 aagggcgaca ttaagcatgc cctgcgcctg aaggacggcg gccggtacct ggcggacttc
3601 aagaccacct acagggccaa gaagcccgtg cagatgcccg gcgccttcaa catcgaccgc
3661 aagttggaca tcacatccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc
3721 gtggcccgcc actccaccgg cggctccggt ggctcctaaa gcggccgcga ctctagatca
3781 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
3841 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
3901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
3961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc
4021 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
4081 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
4141 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
4201 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
4261 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
4321 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
4381 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
4441 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
4501 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
4561 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
4621 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
4681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
4741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac ca
//
pCytERM-mScarlet3-H (aka mYongHong)载体图谱质粒图谱pdf版和pCytERM-mScarlet3-H (aka mYongHong)载体序列质粒序列等相关资料下载
pCytERM-mScarlet3-H (aka mYongHong)质粒载体应用举例
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